Review



custom designed deltagene assay primer pairs  (fluidigm)


Bioz Verified Symbol fluidigm is a verified supplier
Bioz Manufacturer Symbol fluidigm manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 93
      Buy from Supplier

    Structured Review

    fluidigm custom designed deltagene assay primer pairs
    Single HV + Cells Are Totipotent in the Ground State, Related to <xref ref-type=Figure 4 Single cell qRT-PCR was carried out on a BioMark HD System (Fluidigm) using a 96 × 96 chip. DELTAgene Assay custom designed primers (Fluidigm) were used for amplification. Technical replicates were generated for each single cell. (A) Histograms showing the distribution of expression of 4 genes in each population. Data shown unnormalized. (B) Single cells were sorted by flow cytometry into LIF withdrawal conditions. After 7 days differentiated colonies were immunostained for GATA6, CDX2 and BRACHYURY. Quantification of marker expression patterns. 2 and 3 marker exclusive expression indicates colonies that contain independent cells positive for different lineage markers. (C) Whole-mount immunostaining of an E6.5 chimeric embryo generated by injection of a single HV+ ES cell constitutively expressed H2B-Tomato, previously cultured in 2i/LIF, into a morula stage wild-type embryo. Upper whole-mount image shows an extended focus view of the entire embryo. Panels below show optical sections, generated by confocal microscopy, with ES cells contributing to trophoblast and visceral and parietal endoderm (VE and PE, respectively). (D) E6.5 chimeras generated by injection of a single HV- cell that had been cultured in 2i/LIF. The majority of single cells contributed only to epiblast (11/13), with only 2 showing extraembryonic contribution. " width="250" height="auto" />
    Custom Designed Deltagene Assay Primer Pairs, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/custom+designed+deltagene+assay+primer+pairs/pmc03701323-74-11-17?v=fluidigm
    Average 93 stars, based on 32 article reviews
    custom designed deltagene assay primer pairs - by Bioz Stars, 2026-07
    93/100 stars

    Images

    1) Product Images from "Totipotent Embryonic Stem Cells Arise in Ground-State Culture Conditions"

    Article Title: Totipotent Embryonic Stem Cells Arise in Ground-State Culture Conditions

    Journal: Cell Reports

    doi: 10.1016/j.celrep.2013.04.034

    Single HV + Cells Are Totipotent in the Ground State, Related to <xref ref-type=Figure 4 Single cell qRT-PCR was carried out on a BioMark HD System (Fluidigm) using a 96 × 96 chip. DELTAgene Assay custom designed primers (Fluidigm) were used for amplification. Technical replicates were generated for each single cell. (A) Histograms showing the distribution of expression of 4 genes in each population. Data shown unnormalized. (B) Single cells were sorted by flow cytometry into LIF withdrawal conditions. After 7 days differentiated colonies were immunostained for GATA6, CDX2 and BRACHYURY. Quantification of marker expression patterns. 2 and 3 marker exclusive expression indicates colonies that contain independent cells positive for different lineage markers. (C) Whole-mount immunostaining of an E6.5 chimeric embryo generated by injection of a single HV+ ES cell constitutively expressed H2B-Tomato, previously cultured in 2i/LIF, into a morula stage wild-type embryo. Upper whole-mount image shows an extended focus view of the entire embryo. Panels below show optical sections, generated by confocal microscopy, with ES cells contributing to trophoblast and visceral and parietal endoderm (VE and PE, respectively). (D) E6.5 chimeras generated by injection of a single HV- cell that had been cultured in 2i/LIF. The majority of single cells contributed only to epiblast (11/13), with only 2 showing extraembryonic contribution. " title="... System (Fluidigm) using a 96 × 96 chip. DELTAgene Assay custom designed primers (Fluidigm) were used for ..." property="contentUrl" width="100%" height="100%"/>
    Figure Legend Snippet: Single HV + Cells Are Totipotent in the Ground State, Related to Figure 4 Single cell qRT-PCR was carried out on a BioMark HD System (Fluidigm) using a 96 × 96 chip. DELTAgene Assay custom designed primers (Fluidigm) were used for amplification. Technical replicates were generated for each single cell. (A) Histograms showing the distribution of expression of 4 genes in each population. Data shown unnormalized. (B) Single cells were sorted by flow cytometry into LIF withdrawal conditions. After 7 days differentiated colonies were immunostained for GATA6, CDX2 and BRACHYURY. Quantification of marker expression patterns. 2 and 3 marker exclusive expression indicates colonies that contain independent cells positive for different lineage markers. (C) Whole-mount immunostaining of an E6.5 chimeric embryo generated by injection of a single HV+ ES cell constitutively expressed H2B-Tomato, previously cultured in 2i/LIF, into a morula stage wild-type embryo. Upper whole-mount image shows an extended focus view of the entire embryo. Panels below show optical sections, generated by confocal microscopy, with ES cells contributing to trophoblast and visceral and parietal endoderm (VE and PE, respectively). (D) E6.5 chimeras generated by injection of a single HV- cell that had been cultured in 2i/LIF. The majority of single cells contributed only to epiblast (11/13), with only 2 showing extraembryonic contribution.

    Techniques Used: Quantitative RT-PCR, Amplification, Generated, Expressing, Flow Cytometry, Marker, Immunostaining, Injection, Cell Culture, Confocal Microscopy



    Similar Products

    custom designed deltagene assay primer pairs  (fluidigm)
    93
    fluidigm custom designed deltagene assay primer pairs
    Single HV + Cells Are Totipotent in the Ground State, Related to <xref ref-type=Figure 4 Single cell qRT-PCR was carried out on a BioMark HD System (Fluidigm) using a 96 × 96 chip. DELTAgene Assay custom designed primers (Fluidigm) were used for amplification. Technical replicates were generated for each single cell. (A) Histograms showing the distribution of expression of 4 genes in each population. Data shown unnormalized. (B) Single cells were sorted by flow cytometry into LIF withdrawal conditions. After 7 days differentiated colonies were immunostained for GATA6, CDX2 and BRACHYURY. Quantification of marker expression patterns. 2 and 3 marker exclusive expression indicates colonies that contain independent cells positive for different lineage markers. (C) Whole-mount immunostaining of an E6.5 chimeric embryo generated by injection of a single HV+ ES cell constitutively expressed H2B-Tomato, previously cultured in 2i/LIF, into a morula stage wild-type embryo. Upper whole-mount image shows an extended focus view of the entire embryo. Panels below show optical sections, generated by confocal microscopy, with ES cells contributing to trophoblast and visceral and parietal endoderm (VE and PE, respectively). (D) E6.5 chimeras generated by injection of a single HV- cell that had been cultured in 2i/LIF. The majority of single cells contributed only to epiblast (11/13), with only 2 showing extraembryonic contribution. " width="250" height="auto" />
    Custom Designed Deltagene Assay Primer Pairs, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/custom+designed+deltagene+assay+primer+pairs/pmc03701323-74-11-17?v=fluidigm
    Average 93 stars, based on 1 article reviews
    custom designed deltagene assay primer pairs - by Bioz Stars, 2026-07
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    Single HV + Cells Are Totipotent in the Ground State, Related to <xref ref-type=Figure 4 Single cell qRT-PCR was carried out on a BioMark HD System (Fluidigm) using a 96 × 96 chip. DELTAgene Assay custom designed primers (Fluidigm) were used for amplification. Technical replicates were generated for each single cell. (A) Histograms showing the distribution of expression of 4 genes in each population. Data shown unnormalized. (B) Single cells were sorted by flow cytometry into LIF withdrawal conditions. After 7 days differentiated colonies were immunostained for GATA6, CDX2 and BRACHYURY. Quantification of marker expression patterns. 2 and 3 marker exclusive expression indicates colonies that contain independent cells positive for different lineage markers. (C) Whole-mount immunostaining of an E6.5 chimeric embryo generated by injection of a single HV+ ES cell constitutively expressed H2B-Tomato, previously cultured in 2i/LIF, into a morula stage wild-type embryo. Upper whole-mount image shows an extended focus view of the entire embryo. Panels below show optical sections, generated by confocal microscopy, with ES cells contributing to trophoblast and visceral and parietal endoderm (VE and PE, respectively). (D) E6.5 chimeras generated by injection of a single HV- cell that had been cultured in 2i/LIF. The majority of single cells contributed only to epiblast (11/13), with only 2 showing extraembryonic contribution. " width="100%" height="100%">

    Journal: Cell Reports

    Article Title: Totipotent Embryonic Stem Cells Arise in Ground-State Culture Conditions

    doi: 10.1016/j.celrep.2013.04.034

    Figure Lengend Snippet: Single HV + Cells Are Totipotent in the Ground State, Related to Figure 4 Single cell qRT-PCR was carried out on a BioMark HD System (Fluidigm) using a 96 × 96 chip. DELTAgene Assay custom designed primers (Fluidigm) were used for amplification. Technical replicates were generated for each single cell. (A) Histograms showing the distribution of expression of 4 genes in each population. Data shown unnormalized. (B) Single cells were sorted by flow cytometry into LIF withdrawal conditions. After 7 days differentiated colonies were immunostained for GATA6, CDX2 and BRACHYURY. Quantification of marker expression patterns. 2 and 3 marker exclusive expression indicates colonies that contain independent cells positive for different lineage markers. (C) Whole-mount immunostaining of an E6.5 chimeric embryo generated by injection of a single HV+ ES cell constitutively expressed H2B-Tomato, previously cultured in 2i/LIF, into a morula stage wild-type embryo. Upper whole-mount image shows an extended focus view of the entire embryo. Panels below show optical sections, generated by confocal microscopy, with ES cells contributing to trophoblast and visceral and parietal endoderm (VE and PE, respectively). (D) E6.5 chimeras generated by injection of a single HV- cell that had been cultured in 2i/LIF. The majority of single cells contributed only to epiblast (11/13), with only 2 showing extraembryonic contribution.

    Article Snippet: For this experiment, a BioMark HD System (Fluidigm) was used with custom designed DELTAgene Assay primer pairs (Fluidigm; sequences are provided in ).

    Techniques: Quantitative RT-PCR, Amplification, Generated, Expressing, Flow Cytometry, Marker, Immunostaining, Injection, Cell Culture, Confocal Microscopy